Source:羧基磁珠Author:admin Addtime:2018/12/26 Click:
第一章 简介................................................................................................................4
第二章 产品介绍..........................................................................................................7
2.1陶瓷羟基磷灰石 ...........................................................................................7
2.2 纯度 .................................................................................................................9
第三章 羟基磷灰石层析............................................................................................10
3.1 CHT 羟基磷灰石机制 ....................................................................................10
3.2 CHT 在纯化过程中化学变化 ........................................................................11
3.2.1 CHT 化学反应 ......................................................................................11
3.2.2缓冲液中阳离子浓度对于 CHT 表面化学性质的影响 ...................12
3.3 方法指导 .......................................................................................................13
3.3.1 化学兼容性 .........................................................................................13
3.3.2 洗脱 ..................................................................................................... 14
3.3.3 Co-Buffers 使用.....................................................................................15
3.3.4 SNS ........................................................................................................16
3.3.5 钙离子 .................................................................................................17
3.3.6 磷酸根 .................................................................................................17
3.3.7 微量金属元素污染 .............................................................................18
3.3.8 磷酸盐制备 .......................................................................................18
3.3.9 其他清洗 .............................................................................................19
3.3.10 工艺开发 IA:lgG................................................................................20
3.3.11 工艺开发 IB：lgG .............................................................................20
3.3.12 工艺开发：磷酸盐洗脱 ...................................................................21
3.3.13 工艺开发：质粒 ...............................................................................21
3.3.14 工艺开发：酸性蛋白 .......................................................................22
3.3.15 条件摸索 ...........................................................................................22
3.3.16 工艺优化 ...........................................................................................23
第四章 再生，灭菌和贮藏........................................................................................24
4.1 再生 ...............................................................................................................24
4.2 灭菌 ...............................................................................................................24
4.3 贮藏 ...............................................................................................................24
第五章 装柱................................................................................................................25
5.1 一般原则 .......................................................................................................26
5.2.1 推荐的装柱缓冲液 .............................................................................28
5.2.2 搅拌 ..................................................................................................... 28
5.2.3 填料传送 .............................................................................................29
5.3 开放式柱子装柱 ...........................................................................................31
5.4 密闭层析柱装填 ...........................................................................................33
5.4.1 介质转送工作站 .................................................................................33
5.4.2 吸入式填料传送 .................................................................................35
5.4.3卸柱 ...................................................................................................36
5.5 装柱工作站 ...................................................................................................37
5.6 柱效测试 .......................................................................................................39
5.7 装柱 ...............................................................................................................42
5.7.1 对于可以调节柱头的柱子 .................................................................42
5.7.2 对于靠压力的密闭柱子 .....................................................................43
5.7.4 调节柱子进行工艺开发 .....................................................................43
5.8 实验室规模装柱 ...........................................................................................44
5.8.1 Millipore Vantage L 柱子 .....................................................................44
5.8.2 Econo 柱装柱........................................................................................47
第六章 实例分析........................................................................................................49
6.1 装柱—GE Chromaflow 900/200-400.............................................................49
6.2 装柱-Prototype Millipore IsoPak IP P350/500...............................................51
附录..............................................................................................................................54
A1 CHT 与 缓冲液表............................................................................................54
A2 EDTA 络合滴定测定 CHT 流出液中钙离子浓度 ...........................................60
A3 参考文献 ........................................................................................................65
A4 其他资料 ........................................................................................................65
A5 订货信息 ........................................................................................................65
A6 常见问题 ........................................................................................................66
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